Thrombolytic

Efforts have been spend for discovering drugs from medicinal plants. Hibiscus sabdariffa plant a well known plant with many traditional uses for treating different aliments have been selected for canvassing pharmacological activities expressed by the different polarity extracts. Results showed significant antidiabetic activity at different concentrations of glucose solution (5, 10 and 20 mM) (p value < 0.001). Significant moderate anti-inflammatory activity comprising in membrane stabilizing activity were expressed by ethanolic extract ranging (29.573±0.709 - 55.040 ±1.659 %) (p value < 0.001) while mild activity were expressed by petroleum ether extract ranging (10.273±0.413 - 22.347± 1.049 %). Approximately similar thrombolytic activity was exhibited by both extracts ranging (56.663±1.3139-21.533±0.656) consider as significant activity in comparison to the control negative (p value < 0.0001). Results conclude canvass the pharmacological activities expressed by different polarity extracts which were showed significant activity in some aspects which may open new venue for production of new drugs derived from nature with less side effects.

The crude methanol extract of leaf of Tiliacora acuminata Miers as well as organic solvent (n-hexane, carbon tetrachloride and chloroform) fractions and methanolic fraction (aqueous) were subjected to screening for total phenolic content, antioxidant, cytotoxic, thrombolytic and antimicrobial activity. In total phenolic content analysis, the n-hexane soluble fraction of leaf was found to contain highest amount of phenolic content (TPC, 132.18 mg/gm of dry weight of extract, expressed as gallic acid equivalents). In the DPPH assay, the n-hexane soluble fraction of leaf displayed the highest free radical scavenging activity with IC50 value 64.54 μg/ml as compared to 27.5 μg/ml produced by butylated hydroxyl toluene.  A positive correlation was evident between total phenolic content and free radical scavenging activity of T. acuminata having correlation coefficient (R2) of 0.91. In cytotoxicity screening, the crude methanol extract of leaf demonstrated strong cytotoxic activity with LC50 value of 2.40 μg/ml as compared to 0.451 μg/ml produced by vincristine sulphate. During assay for thrombolytic activity, the crude methanol extract revealed 32.5% lysis of clot while standard streptokinase and water, used as positive and negative controls, demonstrated 70.4% and 3.62% lysis of clot, respectively. In antimicrobial assay by disc diffusion method, all the samples exhibited moderate to significant antimicrobial activity (zone of inhibition = 10.0-20.0 mm) against all the test organisms. Among all the samples, the carbon tetrachloride soluble fraction displayed strong antimicrobial activity against Escherichia coli (20.0 mm).