Spectrophotometric determination

Simple, sensitive and accurate spectrophotometric method  has been developed for sequential separation of Co II,NiII and PbII with α-oximinoacetoacetaanilidebenzoylhydrazone(HINABH). HINABH forms Yellowish brown  complex with Co II and yellow colored complexes with NiII and PbII.CoII-HINABH complex was extracted in to n-butanol  in the pH range 6.0-7.0 at λ max 425 nm., whereas Ni II and PbII complexes were extracted into isoamylalcohol quantitatively in the pH range 8.5-9.5 at λ max 420nm and 410 nm respectively. HINABH complexes of Co II,NiII and PbII obeyed Beer’s law in the range of 0.2-5.0 ppm,1-14 ppm and 2-16 ppm respectively. The method had been successfully employed for the determination of Co II,NiII and PbII in the synthetic mixtures and alloys.

Simple, sensitive and accurate spectrophotometric method was proposed for the micro determination of molybdate(MoO42-) using Tannic acid as a reagent. The method was based on the formation of 1:2 orange colored complex between the anion and TA. The optimum conditions for the determination were established. The Beer’s law was applicable in the range of 0.2-5.0 µg/cm3. The method detection limit, limit of quantification, molar absorptivity, Sandell’s sensitivity, coefficient of correlation (β) were reported. The method is free from common interferences. The method was successfully applied for the determination of Mo (VI) in environmental water samples and pharmaceutical products.