Simultaneous equations method

The present manuscript describes simple, sensitive, rapid, accurate, precise and economical spectrophotometric method for the simultaneous determination of Eperisone hydrochloride and Aceclofenac sodium in mixture. The method is based on the simultaneous equations for analysis of both the drugs using methanol as solvent. Eperisone hydrochloride has absorbance maxima at 255 nm and Aceclofenac sodium has absorbance maxima at 275 nm in methanol. The linearity was obtained in the concentration range of 4-20 μg/ml and 4-20 μg/ml for Eperisone hydrochloride and Aceclofenac sodium, respectively. The concentrations of both drugs in synthetic mixture were determined by using simultaneous equations. The mean recovery was 100.34 ± 0.27 and 100.64 ± 0.71 for Eperisone hydrochloride and Aceclofenac sodium, respectively. The method was applied for the determination of these drugs in mixture. The suitability of this method for the quantitative determination of Eperisone hydrochloride and Aceclofenac sodium was proved by evaluating different validation parameters. The proposed method was found to be simple and sensitive for the routine estimation of these drugs in combination. The results of analysis have been validated statistically and by recovery studies.  

The present manuscript describes simple, sensitive, rapid, accurate, precise and economical spectrophotometric method for the simultaneous determination of Gatifloxacin sesquihydrate and Prednisolone Acetate in  mixture. The method is based on the simultaneous equations for analysis of both the drugs using methanol as solvent.Gatifloxacin sesquihydrate has absorbance maxima at 293 nm and Prednisolone Acetate has absorbance maxima at 243 nm in methanol. The linearity was obtained in the concentration range of 2-12 μg/ml and 2-24 μg/ml for Gatifloxacin sesquihydrate and Prednisolone Acetate, respectively. The concentrations of the drugs were determined by using simultaneous equations at both the wavelengths. The mean recovery was 99.19 ± 0.22 and 99.64 ± 0.37 for Gatifloxacin sesquihydrate and Prednisolone Acetate, respectively. The method was successfully applied to laboratory prepared synthetic mixture because no interference from the mixture excipients was found. The suitability of this method for the quantitative determination of Gatifloxacin sesquihydrate and Prednisolone Acetate was proved by validation. The proposed method was found to be simple and sensitive for the routine quality control application of  Gatifloxacin sesquihydrate and Prednisolone Acetate in combination. The results of analysis have been validated statistically and by recovery studies.