Spectrophotometric Method for Simultaneous Estimation of Gatifloxacin Sesquihydrate and Prednisolone Acetate in Combined Pharmaceutical Dosage form

The present manuscript describes simple, sensitive, rapid, accurate, precise and economical spectrophotometric method for the simultaneous determination of Gatifloxacin sesquihydrate and Prednisolone Acetate in  mixture. The method is based on the simultaneous equations for analysis of both the drugs using methanol as solvent.Gatifloxacin sesquihydrate has absorbance maxima at 293 nm and Prednisolone Acetate has absorbance maxima at 243 nm in methanol. The linearity was obtained in the concentration range of 2-12 μg/ml and 2-24 μg/ml for Gatifloxacin sesquihydrate and Prednisolone Acetate, respectively. The concentrations of the drugs were determined by using simultaneous equations at both the wavelengths. The mean recovery was 99.19 ± 0.22 and 99.64 ± 0.37 for Gatifloxacin sesquihydrate and Prednisolone Acetate, respectively. The method was successfully applied to laboratory prepared synthetic mixture because no interference from the mixture excipients was found. The suitability of this method for the quantitative determination of Gatifloxacin sesquihydrate and Prednisolone Acetate was proved by validation. The proposed method was found to be simple and sensitive for the routine quality control application of  Gatifloxacin sesquihydrate and Prednisolone Acetate in combination. The results of analysis have been validated statistically and by recovery studies.