Rutin

Ethanol-induced memory impairment in rats is a consequence of changes within the central nervous system that are secondary to impaired oxidative stress and cholinergic dysfunction. Treatment with antioxidants and cholinergic agonists are reported to produce beneficial effects in animal models. Rutin is reported to exhibit antioxidant effect and cholinesterase (ChE) inhibitor activity. However, no report is available on the influence of rutin on ethanol-induced memory impairment. Therefore, we tested its influence against cognitive dysfunction in ethanol-induced rats using Morris water maze test and Novel object recognition test. Lipid peroxidation and glutathione levels as parameter of oxidative stress and ChE activity as a marker of cholinergic function were assessed in the cerebral cortex and hippocampus. Forty five days after ethanol treated rats showed a severe deficit in learning and memory associated with increased lipid peroxidation, decreased glutathione, and elevated ChE activity. In contrast, chronic treatment with rutin (20-80 mg/kg, p.o., once a day for 45 days) and vitamin C (100 mg/kg, p.o.) improved cognitive performance, and lowered oxidative stress and ChE activity in ethanol treated rats. In conclusion, the present study demonstrates that treatment with rutin prevents the changes in oxidative stress and ChE activity, and consequently memory impairment in ethanol treated rats.

Our health is influenced by many factors, called the determinants of health. WHO has defined its determinants as the set of "personal, social, economic and environmental factors that determine the health status of individuals or populations" (WHO, 1999). Conceptual models or theoretical frameworks help us to understand the complex issues that impede the determinants of health, support health planning interventions and policy development, and most importantly, understand the dimensions of health in order to act to reduce health inequalities between different population groups. This study examined diachronically different frameworks related to the determinants of health, classified them into forty-one conceptual frameworks developed by different countries, organizations and theorists and described as explanatory frameworks, and/or interactive frameworks, and/or action-oriented frameworks. Each framework is detailed according to the important elements and its field of interest. Five frameworks were selected after an in-depth analysis to agree on a comprehensive framework adapted to the Moroccan context and current political concerns and knowledge. In this perspective, our project proposes a conceptual framework of health determinants specific to the Moroccan context that is based on a holistic and intersectoral approach, which recognizes social inequalities in health, describes the role of individuals and communities, gives the importance of upstream action, and helps to improve our understanding of complex problems in the Moroccan health system.

Ethanol-induced memory impairment in rats is a consequence of changes within the central nervous system that are secondary to impaired oxidative stress and cholinergic dysfunction. Treatment with antioxidants and cholinergic agonists are reported to produce beneficial effects in animal models. Rutin is reported to exhibit antioxidant effect and cholinesterase (ChE) inhibitor activity. However, no report is available on the influence of rutin on ethanol-induced memory impairment. Therefore, we tested its influence against cognitive dysfunction in ethanol-induced rats using Morris water maze test and Novel object recognition test. Lipid peroxidation and glutathione levels as parameter of oxidative stress and ChE activity as a marker of cholinergic function were assessed in the cerebral cortex and hippocampus. Forty five days after ethanol treated rats showed a severe deficit in learning and memory associated with increased lipid peroxidation, decreased glutathione, and elevated ChE activity. In contrast, chronic treatment with rutin (20-80 mg/kg, p.o., once a day for 45 days) and vitamin C (100 mg/kg, p.o.) improved cognitive performance, and lowered oxidative stress and ChE activity in ethanol treated rats. In conclusion, the present study demonstrates that treatment with rutin prevents the changes in oxidative stress and ChE activity, and consequently memory impairment in ethanol treated rats.

An cerebroprotective phytoconstituent loaded niosomes for nose to brain delivery was formulated. The main objective of this study was firstly to enhance the solubility and bioavailability of poorly soluble phytoconstituents Rutin and Quercetin, secondly to ease the administration through nasal route and thirdly to investigate the niosome encapsulated phytoconstituent for its cerebroprotective activity. Method: Niosomal formulations were optimized using design of experiment by altering the proportions of range of non-ionic surfactants(Tween 80, Pluronic L81, PluronicP123, span80,captex 200P,Capmul PG NF8,Labrasol), Ratio of cholesterol to surfactant, RPM and sonication. The formulations were prepared by ether injection method. The formulation was then evaluated for morphological characterization, encapsulation efficiency, and viscosity. Cerebroprotective activity of optimized niosomal formulation against bilateral carotid artery occlusion (BCAO) induced stroke in rats was studied. Phytoconstituent niosomes formulated using Tween 80 was found to entrap high amounts of drug, and show sufficient quantity of in vitro release.  Consequences showed that the niosomal formulation of Rutin and Quercetin gives the cerebroprotective action at the dose of 3mg/kg of each drug. Based on the results of histopathology study, it can be concluded that test formulation may have potential to attenuate histopathological alterations caused due to ischemia and can be considered as a promising approach for the cerebroprotective Phytoconstituents.

Development of amphiphilic drug-lipid complexes is a potential approach for improving delivery of the drugs by increasing solubility, release profile and oral bioavailability. Rutin, a polyphenolic flavonoid, shows several biological effects like capillary protectant, anti-oxidant, anti-inflammatory, anti-aging, cardio-protective, anti-thrombotic and neuroprotective, but its use is limited due to its low aqueous solubility. To overcome this limitation, phospholipid complex of Rutin was developed to improve its aqueous solubility for better absorption through the gastrointestinal tract and this might result in improved bioavailability. The Rutin phytosomes prepared by solvent evaporation method using different ratios of Rutin and Soybean phosphatidylcholine (1:1, 1:2 and 1:3) was evaluated for percentage yield, compatibility studies by infra-red spectroscopy, particle size, poly dispersity index, zeta potential, drug content and were found to be within the acceptable range. Surface morphology by scanning electron microscopy, solubility studies, in-vitro drug release and stability studies also were carried out. The phospholipid complex of Rutin was found to be fluffy and porous with rough surface. The water solubility of Rutin was improved from 0.058mg/ml to 0.475 mg/ml in the prepared Phytosomes. The in-vitro drug release studies showed that there is no drug release from pure drug and F1 formulation up to 120min in acidic buffer pH 1.2; while in phosphate buffer pH 7.4 showed releases about 49.3% and 92.85% respectively, which indicates the significant enhancement of dissolution of Rutin phytosomes compared to pure drug. Stability studies suggested that the formulations were stable. In this study, Phytosomes could be successfully tailored for Rutin with improved dissolution characteristics which is promising for lowering the influence of exogenous factors and increasing drug delivery.