Rifampicin

The objective of the study was preparation of sustained release matrix tablets by hot melt extrusion (HME) and process optimization for continuous manufacturing. Furthermore, HME tablets were evaluated with respect to in vitro release rate, erosion behavior and water uptake study. Hydroxypropylcellulose (HPC) was used as release retarding polymer. The drug chosen for study was first line anti tuberculosis drug rifampicin. 50 % drug loaded HME tablets were prepared. The HME tablets were characterized by DSC, FTIR and SEM. No chemical interaction was found between drug and polymer as per DSC and FTIR. Dispersion of drug particles and internal micro pores was observed by SEM. In vitro release study revealed 50% drug loaded HPC matrix tablets gave sustained release of 101.41±1.02% at the end of 24 h. Release rate of rifampicin from HME tablets was found to be dependent on the concentration of polymers and plasticizer. The release rate from the edges and circumference of tablets suggested the hydration of the tablets from the circumference was more significant than at edges of the tablets. Drug release from HME matrix tablets was found to follow Super case-II transport mechanism. HME tablets were stable for six months as per ICH guideline.

The hepatoprotective effects of Methanolic Extract of Justicia Beddomei (MEJB)on Isoniazid and Rifampicin (INH and RIF) induced hepatotoxicity and the probable mechanism(s) involved in this protection were investigated in rats. Male wistar rats were divided in to five groups and used for the study. Hepatotoxicity was induced in animal models and was compared with the protective effect of standard drug Silymarin. In the present study it was noted that the administration of INH, Rifampicin decreased the level of total protein, albumin and increased the level of liver enzymes AST, ALT, ALP and TC. These parameters were brought back to the near normal level in control group treated with MEJB. The histopathological studies also revealed that treatment with Methanolic Extract of Justicia Beddomei (MEJB) showed a protection against injuries due to the effect of INH, Rifampicin. These results suggets that MEJB showed significant hepatoprotective activity against Isoniazid and Rifampicin induced hepatotoxicity and this might be due to the presence of flavanoids and phenolics. Further research is sought to explore the mechanism of action and phytoconstituents responsible for the pharmacological action.

  The Aim of this study was preparation of dry powder formulation of rifampicin loaded polymeric microparticles as dry powder formulation for inhalation in effective tuberculosis treatment.The microparticles containing rifampicin (RIF) were prepared by spray drying method using different biocompatible polymers like chitosan and hydroxyl propyl methyl cellulose (HPMC) The microparticles and microparticle blend with coarse carrier Inhalac 230 were investigated for its aerosolization properties like emitted dose, Mass median aerodynamic diameter, Fine particle Fraction, Geometric Standard Deviation.The spray drying method produced wrinkle surfaced porous microparticles under the size range of 10µm. Mass median aerodynamic diameter obtained for all formulation ranged in 2.68 µm to 3.73 µm and Fine particle fraction in between 51.58 ± 5.36 to 72.74 ± 3.18. The lowest tapped density value obtained was 0.102 g/cm2 belong to formulation coded M1. In vitro deposition studies using cascade impactor showed emitted dose of > 90% for all batches. The polymeric microparticles produced by spray drying technique showed promising particle characteristics suitable for inhalation with Fine particle fraction (72.74 ± 3.18) of total emitted dose, after blending with lactose. The blending of the microparticles with Inhalac 230 allowed the Fine particle fraction values to increase by increasing the dispersibility of powder on inspiration. Key words: Rifampicin, Dry Powder Inhalation, Chitosan, HPMC, Interactive blend.

The present manuscript describe simple, sensitive, rapid, accurate, precise and cost effective dual wavelength spectrophotometric method for the simultaneous determination of Rifampicin and Piperine in combined capsule dosage form. The utility of dual wavelength data processing program is its ability to calculate unknown concentration of components of interest in a mixture containing an interfering component. The principle for dual wavelength method is “the absorbance difference between two points on the mixture spectra is directly proportional to the concentration of the component of interest”. The method was based on determination of Rifampicin at the absorbance difference between 286 nm and 357 nm and Piperine at the absorbance difference between 356 nm and 479 nm. The linearity was obtained in the concentration range of 10-60 μg/ml for Rifampicin and 1-10 μg/ml for Piperine. The mean recovery was 98.40 ± 0.48 and 98.59 ± 0.46 for Rifampicin and Piperine, respectively. The method was successfully applied to pharmaceutical dosage form because no interference from the capsule excipients was found. The suitability of these methods for the quantitative determination of Rifampicin and Piperine was proved by validation. The proposed methods were found to be simple and sensitive for the routine quality control application of Rifampicin and Piperine in pharmaceutical capsule dosage form. The results of analysis have been validated statistically and by recovery studies.