Phytochemical analysis

The present study was carried out to find out the antimicrobial activity and phytochemical analysis of Mint (Mentha arvensis) leaves solvent extracts against selected enteric pathogens. Plants have been an important source of medicine with qualities for thousands of years. In the present study, the antimicrobial efficacy of Mint leaves solvent extracts was examined using Ethanol, Methanol, Diethyl ether and Acetone as solvents and tested against nine enteric pathogens namely E.coli, Salmonella typhi, Sal.Para.A, Sal. Para. B, Shigella sonnei, Shigella dysentarie, Enterobactor spp.,Citrobactor spp.and Klebsiella spp. Antimicrobial analysis was done by using agar well diffusion method against selected enteric pathogens. The MIC values were determined by both agar and broth dilution method. The functional chemical group was determined by Fourier Transform Infrared Spectroscopy (FTIR).The Ethanol extract of Mint showed highest antimicrobial activity against Shigella sonnei., while lowest antimicrobial activity was observed by Ethanol extract of Mint against Citrobactor spp and by Acetone extract of Mint against Shigella dysentarie .The phytochemical analysis of Mint leaves solvent extracts showed presence of alkaloid, flavonoid, phenol, tannin, glycosides etc.

Cyperus rotundus Linn. (Family: Cyperaceae), commonly known as Mothais a multivalent plant widely used in traditional medicine around the world for treatment of various diseases (fever, dysentery, malaria, skin disease, diarrhoea etc.). The present study was carried out to investigate the phytochemical profile of leaves of Cyperus rotundus. The powder of the leaves of Cyperus rotundus was successively extracted with acetone, aqueous, benzene, chloroform, cyclohexane, ethylacetate and methanol. A number of biologically active compounds have been isolated from the plant. Phytochemical analysis shows the presence of carbohydrates, glycosides, proteins, amino acids, phenolic compounds, tannins, steroids, anthraquinone, anthocyanin, flavonoid, saponins and alkaloid. Our findings provided evidence that crude aqueous and organic solvent extracts of these tested plants contain medicinally important bioactive compounds and it justifies their use in the traditional medicines for the treatment of different diseases. The result of the study could be useful to description and foundation of monograph of the plant. Key words:  Phytochemical analysis, Saponins, Flavonoids and Cyperus rotundus.