MBTH

The target of the present study is to use experimental design in screening and optimizing experimental variables to develop a spectrophotometric method for determination of Cefdinir (CFN) antibiotic. The proposed method is based on the oxidative coupling reaction of CFN with 3-methylbenzothiazolinone-2- hydrazone (MBTH) in presence of FeCl3 in acidic medium forming a green colored chromogen measured at λ max of 660 nm. A 25-2 fractional factorial design was utilized to screen the effect of FeCl3, HCl and MBTH concentrations, in addition to reaction time and nature of diluting solvent on the absorbance of the formed chromogen. One way ANOVA and Pareto ranking analyses have shown that all variables were statistically significant. Full factorial design was then utilized to evaluate the effects of the variables on the selected response. With the help of a response surface and contour plots the optimum value of each variable was determined and used for further experiments. These optimum values were 5mg/mL FeCl3, 0.06% HCl, 4mg/mL MBTH, reaction time of 10 min and methanol as diluting solvent. Beer’s law is obeyed in the range of 0.5-6.0 μg/mL. The proposed method was successfully applied for the determination of CFN in bulk powder and commercial dosage forms without interference from the commonly encountered excipients and additives. The mean recoveries of the analyte in pharmaceutical preparations were in agreement with those obtained from a comparison method, as revealed by statistical analysis of the obtained results using the Student's t-test and the variance ratio F-test.

  A simple and sensitive visible spectrophotometric method for the determination of tolterodine tartrate using 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH) reagent has been developed in bulk and tablet dosage forms. It is based on the formation of intense blue colored species by treating the drug with MBTH reagent in the presence of ferric chloride with an absorption maximum of 650nm. The Regression analysis of Beer’s Law plot showed good correlation in a general concentration range of 5-25µg/ml. The proposed method is validated with respect to accuracy, precision, linearity and limit of detection. The suggested procedure is successfully applied to the determination of the drug in pharmaceutical preparation, with high percentage of recovery, good accuracy and precision. The results of analysis have been validated statistically by repeatability and recovery studies. The results are found satisfactory and reproducible. The method is applied successfully for the estimation of tolterodine tartrate in capsule form without the interference of excipients.

  3-methylbenzthiazolinone-2(3H)-hydrazone is a chromogenic reagent used in spectrophotometric determination of pharmaceuticals. This review explains the work done by different researchers so far for estimation of the drugs by spectrometric analysis. Therefore, this review gives compiled data on utilisation of MBTH reagent in spectrometric analysis   Key Words: Spectrophotometry, MBTH, Chromogen.