Ferric chloride

A visible spectrophotometric method which is simple, precise and economical has been developed and validated for the estimation of Faropenem in pharmaceutical bulk and tablet dosage form. Identification was carried out using a UV-Visible double beam spectrophotometer with working wavelength at 639.5 nm. It was based on the formation of a colored complex between Faropenem and MBTH (3-Methyl-2-Benzothiazolinone hydrazone hydrochloride) reagent in presence of Ferric chloride. The method was validated with respect to its specificity, linearity range, accuracy, and precision in analytical media. Regression analysis showed good correlation in the concentration range 25-300 μg/ml and the % relative standard deviation as 0.1262. Statistical treatment of data reflects that the proposed method is accurate and easily applicable for determination of Faropenem in bulk and pharmaceutical preparation. The different experimental parameters effecting the development and stability were studied carefully and optimized. A result of analysis for the method was validated statistically and recovery studies were also performed.

  A simple and sensitive visible spectrophotometric method for the determination of tolterodine tartrate using 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH) reagent has been developed in bulk and tablet dosage forms. It is based on the formation of intense blue colored species by treating the drug with MBTH reagent in the presence of ferric chloride with an absorption maximum of 650nm. The Regression analysis of Beer’s Law plot showed good correlation in a general concentration range of 5-25µg/ml. The proposed method is validated with respect to accuracy, precision, linearity and limit of detection. The suggested procedure is successfully applied to the determination of the drug in pharmaceutical preparation, with high percentage of recovery, good accuracy and precision. The results of analysis have been validated statistically by repeatability and recovery studies. The results are found satisfactory and reproducible. The method is applied successfully for the estimation of tolterodine tartrate in capsule form without the interference of excipients.