Gymnema sylvestre

The aim of the present research is anti diabetic activity of polyherbal formulation comparing with individual plant extractions. The anti diabetic activity of a polyherbal formulation was evaluated in using Alloxan ?- cytotoxin induced chemical diabetes in a wide variety of wister rats. Methanolic extract of the poly herbal formulation, prepared from powder of plants Fruits of Momordica charantia, stem and root of Tinospora cordifolia , aerial parts of Andrographis peniculeta and wood of Pterocarpus marsupium and leaves of Gymnema sylvestre  was subjected to phytochemical test and pharmacological screening of Anti diabetic activity at a dose level 400mg/kg. The PHME (400mg/kg) treated diabetic rats show mean (±SEM) fasting serum glucose of 253.11±3.41mg/dl on day 0 which was reduced to 161.43±3.55mg/dl on day 7, 107.45±4.52mg/dl on day 14  . These changes in fasting serum glucose illustrate that the diabetic rats treated with PHME (400mg/kg) show a progressive and significant (p?0.05) reduction in fasting serum glucose during the treatment period in comparison to diabetic group of rats. It was concluded that individual extraction shows the activities but in combination of plants it shows synergistic effect so poly herbal extraction is useful more when compared with given in individual plants.

Aqueous Extract Followed By Hydroalcoholic And Alcoholic Extract Gymnema Sylvestre Roxb.1,2 Family Asclepiadaceae And Stevia Rebaudiana Bertoni3amily Compositae. Literature survey reveals that till date, no combination of Stevia rebaudiana and Gymnema sylvestre are available in any dosage form. In the present work, the main objective is to formulate, evaluate and validate anti-diabetic liquid oral preparation of Gymnema using Stevia as sweetener. Stevia, non-caloric natural sweetener has added advantage than artificial sweetener due to number of beneficial effects Successive solvent extraction of Gymnema sylvestre and Stevia rebaudiana were carried out with the solvents like petroleum ether (60-800C), ethanol, hydroalcohol and water. Phytochemical screening of aqueous extract of formulation was performed and their purity was checked by TLC of aqueous extract of Gymnema sylvestre, formulation and deacylgymnemic acid were done. While preparing liquid oral formulation, aqueous extract of Gymnema sylvestre and Stevia rebaudiana were selected, because of their maximum yield and presence of phytochemicals, which were present in alcoholic and hydroalcoholic extract. Stevia rebaudiana is a natural sweetner. In the presented formulation, stevia was used as a sweetner to make the formulation sweet. Validation of liquid oral preparation was done. Physical, chemical evaluations were carried out. The amount of deacylgymnemic acid present was found to be 22.327 mg/ml by HPLC. 

Gymnema sylvestre has been extensively evaluated for its in vitro and in vivo activity against diabetes mellitus. The leaves of the shrub Gymnema sylvestre contain a complex of pentacyclic triterpenes known as gymnemic acids that have been reported as potential therapeutic agents in the treatment of diabetes. The present study reveals the relationship between the structure and function of the medicinally important constituents of this plant. To understand the binding mechanisms of these active constituents, molecular modelling studies has been performed with dipeptidyl peptidase-4, protein tyrosine phosphatase 1B, sodium potassium ATPase, aldose reductase and glycogen synthase kinase-3β as target proteins using XP docking program of Glide, version 9.2, Schrödinger suit. These constituents showed favourable interactions with the amino acid residues at the active site, their by substantiating their proven efficacy as antidiabetic agents. The present study also gives an insight to the probable herb-drug interaction and reason for sudden hypoglycemic shocks that may occur on concurrent administration of Gymnema extract and synthetic drug, Glimepiride, for the treatment of diabetes.