Gas chromatography

The majority of pharmaceuticals used today are chiral. A brief summary of various chiral separation principles and associated separation methods is provided in this review. Liquid chromatography (LC), capillary electrophoresis (CE), high-performance liquid chromatography (HPLC), and gas chromatography (GC) are only a few of the recent advancements addressed. The creation of competent analytical techniques is essential for compliance with the numerous regulatory obligations. The rapid development of chiral separation technologies has been fueled by the current situation in which chirality factors are taken into account in therapeutic benefit and drug development. High-performance liquid chromatography (HPLC) and gas chromatography (GC) have unquestionably made important advancements in chiral chromatographic technology, opening the door to the quantification of specific enantiomers of a number of racemates in biological fluids such plasma, serum, urine, etc. Chiral compound separations and analyses are crucial in a variety of industries, such as biology, pharmaceutical manufacturing, and the creation of chemical intermediates.

  Residual solvents in intermediates and active pharmaceutical ingredient were monitored by using gas chromatography. It is mandatory to control adequately the quality of active pharmaceutical ingredient by checking the levels of residual solvents. A systematic approach for the identification and quantification of residual solvents in citalopram hydrobromide was described in proposed analytical method. The analysis was carried  on DB624 (30 m x 0.32 mm id, 5 µm  coating thickness) capillary column by gas chromatography with flame ionization detector. It was validated as per ICH guidelines1. The method was validated for system suitability, specificity, LOD, LOQ, linearity, accuracy, precision and robustness. The method described was simple, sensitive, reliable and reproducible for the quantization of residual solvents at levels as per ICH guideline. Key words: Citalopram hydrobromide, Active pharmaceutical ingredient, Residual solvent, Gas chromatography

  This research paper describes simple analytical method for determination of Camylofin dihydrochloride and Paracetamol in syrup formulation by Gas chromatography method. Benzoic acid was used as internal standard. Validation was carried out in compliance with the International Conference on Harmonization guidelines. The method utilized GC (Agilent Technologies 6890N Network GC system with FID detector), and RTX-5 capillary column (Crossbond 50% diphenyl-95% dimethyl polysiloxane), 30m x 0.53mm, 1.5µm as stationary phase. Helium was used as the carrier gas.  The proposed method was validated for linearity, LOD, LOQ, accuracy, precision, ruggedness and solution stability. It can be conveniently adopted for routine quality control analysis. Key Words:Validation, Gas chromatography, Pharmaceutical preparations, Camylofin dihydrochloride, Paracetamol.