FRAP

Coumarin Schiff bases (1-14) synthesized by microwave irradiation method, were evaluated by insilico techniques and for their antioxidant activity by ferric reducing antioxidant power (FRAP) assay. The results from insilico techniques showed that all the compounds exhibit drug likeness satisfying Lipinski rule of five having the ability to behave as drugs. These findings revealed that they are active nuclear receptor, GPCR ligand and enzyme inhibitors. Insilico OSIRIS predictions indicate that all the compounds suffer from teratogenic effect, compounds 9, 14 possesses mutagenic toxicity. OCHEM results clearly indicate that they are inhibitors of CYP1A2 a subtype of cytochrome P450. Compound 8 gave FRAP value nearer to the standard. The study provides a good scope for further development of novel targeted molecules.

A novel antioxidant oxochromen derivative was isolated and characterized from Thespesia populnea stem bark (Linn.) with the evaluation of its in vitro antioxidant activity. The isolated compound was characterized by FTIR, 1H NMR, 13C NMR, and mass spectral technique. The spectral data revealed the structure [(2S,3R,4S,5S,6R)-2-(5,7-dihydroxy-2-methyl-4-oxochromen-6-yl)-4,5-dihydroxy-6-(hydroxyl methyl) oxan-3-yl] 3,4,5-trihydroxybenzoate. Antioxidant activity of the isolated compound was evaluated by DPPH scavenging activity, ABTS scavenging activity and Ferric reducing /antioxidant power assay. Results show that isolated compound from the acetone extract fraction of T. populnea stem bark exhibit better antioxidant activity at lower concentration in all the methods and at higher concentrations they act as prooxidants. In conclusion the present results suggested that the isolated compound could be a potential antioxidant agent for preparing functional foods and nutraceuticals applied in food and pharmaceutical industries.

The study was aimed to determine antioxidant activity of methanolic extracts of both leaf and root of 12 nos. of therapeutically used mangrove species of Odisha coast using different antioxidant assays i.e., DPPH, FRAP and ‘Reducing Power’. Significantly higher DPPH radical scavenging effect was observed in leaf extracts of Heritierafomes (IC50=13µg/ml). However, in root extract, the activity was higher in Rhizophoraapiculata (IC50=17µg/ml). As far as total antioxidant content is concerned, Avicennia marina showed highest content i.e94.16±5.36 mg AEAC/g dry wt.in leaf sample and87.33±0 mg AEAC/g dry wt. in root sample. The highest FRAP value (4.75±0.08 mM AAE/g dry wt.)  in leaf samples was found in Cerberamanghas. Whereas, highest FRAP value (5.19±0.40 mM AAE/g dry wt.) in root extract was noted in Cynometrairipa. As regard to reducing power, Kandeliacandel showed highest activity e.g.,3.49±0.08 mg AAE/g dry wt. in leaf extracts among the studied species. In root extract, Aglaiacucullata exhibited highest reducing activity (3.35±0.01 mg AAE/g dry wt.). This study revealed that H. fomes, R. apiculata, A. marina, C. manghas, C. iripaand K. candelwere found to be good sources of natural antioxidants for pharmaceutical utilization.