Column chromatography

Echinops echinatus Family: Asteraceae is used as medicinal plant used in urinary disorder, liver disorder, heart diseases, etc. The seeds are sweet and aphrodisiac (Aurveda). The methanolic extract of leaves was obtained by Soxhelt extractor followed by concentration in rotary evaporator. Separation of bioactive chemicals was carried out by column chromatography while analysis by GC-MS which shows presence of following chemicals compositions. Phenol, 2,4bis(1,1dimethylethyl), Pentanoic acid, 5hydroxy,2,4ditbutylphenyl esters, 7,9Ditertbutyl1oxaspiro(4,5)deca6,9diene2,8dione, Benzo[b]dihydropyran, Propanoic acid, 2methyl,(dodecahydro 6hydroxy9methyl3methylene2,9dioxoazuleno[4,5b] furan6yl) methyl ester, Hexadecanoic acid,2hydroxy1(hydroxymethyl) ethyl ester, Glycerol 1palmitate, Octadecanoic acid, 2,3dihydroxypropyl ester, Octadecanoic acid,2hydroxy1(hydroxymethyl)ethyl ester, DISTEARIN, 13Docosenamide,(Z), Bis(cis13docosenamido) methane, Stigmasterol, 28,33 Dinorgorgost5en24one,3hydroxy, 3Dodecene,

The present study was to evaluate preliminary phytochemical analysis and in vitro cytotoxic activity on root extracts and fractions of Jatropha gossipiifolia (family Euphorbiaceae). The Chloroform and ethanol extract was obtained by hot continuous percolation by soxhlet apparatus. The phytochemical analysis of both the extracts shows the presence of flavonoids, alkaloids, tannins, phenolic compounds and steroids. Both the extracts subjected to in vitro cytotoxic activity against breast cancer cell line (MCF–7) cell line by MTT assay. The active extract (ethanol) was then subjected to fractionation by column chromatography by gradient elution from n hexane – ethanol. The fractions (chloroform 100%, chloroform: ethyl acetate, ethyl acetate: ethanol, ethanol 100%) The fractions were then subjected to in vitro cytotoxic activity against MCF– 7 cell line by MTT assay. The ethanol extract shows good anticancer activity. The percentage growth of MCF-7 cells in chloroform and ethanol extract treated against MCF-7 cell line was found to be 49.59 and 28.46 respectively. The EC50 value of chloroform and ethanol extract was found to be 0.00018 & 0.00055mg/ml respectively. The ethanol 100% shows good anticancer activity compared to other fractions against MCF-7 cell line with the percentage growth of 14.650 and EC50 value was found to be 0.00087mg/ml.