Extraction and Characterization of Echinops Echinatus Plant Extract by Column Chromatography and GC-MS Analysis

Echinops echinatus Family: Asteraceae is used as medicinal plant used in urinary disorder, liver disorder, heart diseases, etc. The seeds are sweet and aphrodisiac (Aurveda). The methanolic extract of leaves was obtained by Soxhelt extractor followed by concentration in rotary evaporator. Separation of bioactive chemicals was carried out by column chromatography while analysis by GC-MS which shows presence of following chemicals compositions. Phenol, 2,4bis(1,1dimethylethyl), Pentanoic acid, 5hydroxy,2,4ditbutylphenyl esters, 7,9Ditertbutyl1oxaspiro(4,5)deca6,9diene2,8dione, Benzo[b]dihydropyran, Propanoic acid, 2methyl,(dodecahydro 6hydroxy9methyl3methylene2,9dioxoazuleno[4,5b] furan6yl) methyl ester, Hexadecanoic acid,2hydroxy1(hydroxymethyl) ethyl ester, Glycerol 1palmitate, Octadecanoic acid, 2,3dihydroxypropyl ester, Octadecanoic acid,2hydroxy1(hydroxymethyl)ethyl ester, DISTEARIN, 13Docosenamide,(Z), Bis(cis13docosenamido) methane, Stigmasterol, 28,33 Dinorgorgost5en24one,3hydroxy, 3Dodecene,