Carbon tetrachloride

In the present study, hepatoprotective effect of an ethanolic extract of Ipomoea batatas leaves, on liver injury induced by carbon tetrachloride (CCl4) was investigated. Wistar albino rats (n=6) were administered 300, 600 and 900 mg/kg body weight extract orally for 10 consecutive days. Marker enzymes SGOT, SGPT, serum protein and Bilirubin (total, direct and indirect) were estimated in serum whereas total protein (TP), Lipid peroxidation (LPO), superoxide dismutase (SOD) and catalase were estimated in liver tissue as markers for oxidative stress.  Histopathological assessment was also done on liver tissue. Ethanolic extract of Ipomoea batatas leaves administration for 10 days prevented the CCl4 induced hepatic injury and oxidative stress. Hepatoprotective effect of I. batatas in CCl4 induced liver poisoning was evident by decrease in elevated levels of SGOT, SGPT and bilirubin. Treatment with I. batatas increased the reduced levels of TP and SOD and decreased the elevated level of MDA concentration and catalase activity in CCl4 induced oxidative stress in the liver. The ability of ethanolic extract of Ipomoea batatas leaves to protect the liver toxicity in rats was further confirmed by histological findings in the liver tissue. In conclusion, it was observed that ethanolic extract of Ipomoea batatas leaves have a dose dependent potential to protect the liver against CCl4induced hepatic injury through its potent antioxidant activity in rats.

The aim of the present study was to optimize carbon tetrachloride (CCl4­) induced hepatotoxicity in the rat with respect to dose and time course. Female Sprague Dawley rats, weighing 150-200g were used in the present study. Hepatotoxicity was evaluated by measuring the activity of serum enzymes, alanine transaminase [ALT], aspartate transaminase [AST], alkaline phosphatase [ALP] and total bilirubin [TBIL] level. Experimental hepatotoxicity was induced by administering 1, 1.5, 2 ml/kg CCl4 (dissolved in an equal volume of olive oil) intraperitonially (i.p) and observed for enzyme levels at time intervals of 0, 2, 24, 48 hours after CCl4­ challenge. Result values are analyzed by One way ANOVA followed by Dunnett’s test. 1 ml/kg CCl4 increased the levels of serum enzymes that reached a peak after 24 hr and showed moderate hepatitis which is ideal for development of acute hepatotoxicity. It is possible to select optimum dose of CCl4 to induce hepatotoxicity by single i.p injection in order to study hepatoprotective activity of herb without causing death of animals. Keywords- Carbon tetrachloride, Dose, Hepatotoxicity, Time course

The hepatoprotective activity of a Boerrhavia diffusa of 50 % ethanolic extract  was studied using Swiss albino rats. The animals received a single intraperitoneal injection of N-nitrosodiethylamine 200mg/kg body wt followed by subcutaneous injection of CCl4 in a dose of 3 ml/kg body wt. The administration of  Boerrhavia diffusa extracts and cisplatin decreased the liver weight and average liver weight, which shows the rehabilitating capability of extracts in respect with anticancer potency in comparison with the very much effective in preventing NDEA-induced multistage hepatocarcinogenesis possibly through antioxidant and  antigenotoxic nature, which was confirmed by various liver injury and biochemical tumour markers enzymes and molecular events. Boerrhavia diffusa extract dose dependently and significantly the increase in serum hepatic enzyme levels after NDEA& CCl4 treatment compared to the toxin control group. The results of this study confirmed the antioxidant and hepatoprotective activity of the Boerrhavia diffusa extract against carbon tetrachloride& N-nitrosodiethylamine induced hepatotoxicity in Swiss albino rats.