Aluminium chloride

To investigate the effect of Aluminium chloride on the activities of testicular enzymes in rat. Forty male Albino rats were divided into four groups (n=10). Treatment was given for a period of 45 days daily. Group-I Control given only distilled water, Group-II treated with aluminium chloride 50 mg/Kg body wt. Group-III treated with Vitamin E 20mg/Kg body weight. Group-IV treated with aluminium chloride+ Vitamin-E. The animals were sacrificed after 24 hours duration. Biochemical studies were observed in testis. Marker enzymes ALP, ACP and LDH, SDH activities were decreased significantly with control. Lipid peroxidation was increased significantly in aluminium chloride treated rats. The administration of AlCl3+Vit E shows protective effect from the toxicity.

The aerial parts of plant contain a number of medicinally important compounds. The present study was carried out to extract the flavonoid rich fraction from the aerial parts of Hemidesmus indicus Linn. R. Br. and identified the phytochemicals presents in the flavonoid rich fraction of aerial parts of Hemidesmus indicus Linn. R. Br. & determined the total flavonoid contents and antioxidant potential of obtained favonoid rich fraction of Hemidesmus indicus Linn. R. Br. Total flavonoid content was estimated by Aluminium Chloride colorimetric method and the flavonoid content was 0.406 % TFC (Total Flavonoids Contents) in grams of Quercetin equivalent. Antioxidant activity was evaluated by DPPH method and IC50 value of flavonoid rich fraction of aerial parts of Hemidesmus indicus Linn. was found to be 21.39 µg/ml. Key words: Total flavonoids, Aluminium chloride, Antioxidant activity, DPPH, IC50 value.