Achyranthes aspera

A simple, accurate, rapid and precise isocratic High performance liquid chromatographic (HPLC) method was developed and validated for the quantification of betaine in Achyranthes aspera. The method employs Reversed Phase Phenomenex (US) C18 Column 250 x 4.6mm I.D, 5µ Particle Size system consisted of prominence LC-20AT gradient pumps, Prominence SIL- 20A Auto sampler and a Prominence SPD-M20A Diode Array Detector (SHIMADZU), Japan and flow rate of 1.0 ml/min with a load of 20μl. Water and acetonitrile was used as mobile phase in the composition of 10:90. The detection was carried out at 242 nm. Linearity range was 0.32-0.48 mg/ml. Retention time of betaine was found to 8.42 min. The recovery studies 98-103%. This method was validated for accuracy, precision, linearity and Robustness as per ICH guidelines. The method was found to be specific, selective, and robust.

Phytochemicals are dependable sources for the treatment of different health problems. In this study, antioxidant activities of different sequential extracts (petroleum ether, benzene, chloroform, ethyl acetate, ethanol and aqueous) of root and inflorescences of Achyranthes aspera Linn. (Family: Amaranthaceae) was done by different standard methods of phytochemical screening. Our finding reveals that all the extracts of A. aspera root and inflorescences contain alkaloids, tannins, cardiac glycosides, steroids, flavonoids, terpenoids, reducing sugar and saponin in appreciable, moderate and trace amount. Observed result showed that the phytochemical contents are high in different sequential extracts of root than sequential extracts of inflorescences. Due to rich source of phytochemicals, this plant is may be used for herbal medicine.