5

2 (H/substituted)-4-aryl-5H,6H, 7-arylimino-1,2,3,5,6-thiatetraazepines (6) have been obtained by basification of 2-(H/substituted)-4-aryl-5H,6H-7-arylmino-1,2,3,5,6-thiatetraazepine monohydrochloride (5).  The latter were synthesized by the interaction of N-aryl-S-chloro isothiocarbamoyl chloride (4) and 1-(H / substituted)-3-aryl-dihydroformazan (3), which were prepared initially by the condensation of aryl acid hydrazide (1) and hydrazine hydrate or substituted hydrazine (2). Compound (6) on benzoylation with benzoyl chloride and excess 10 % sodium hydroxide solution afforded benzoyl derivatives (7) and on boiling with aqueous ethanolic sodium hydroxide solution isomerizes into corresponding 1, 2, 3, 5, 6-pentaazepines (8).  The structures of all the synthesized compounds were established on the basis of elemental analysis, equivalent weight determination, spectral analysis like IR, 1H-NMR and Mass.  These newly synthesized compounds (6) were screened for their antifungal and antibacterial activity.

The methanol extract of Callistemon salignus was subjected to different phytochemical tests  and gives positive results for glycosides, tannin, carbohydrate, volatile oil, flavonoid and steroid. This extract leads to the isolation of 3′, 4′, 5, 7-tetra-hydroxy flavonol by chromatographic separation through a graded elution. The identity of the isolated compound was checked by preliminary phytochemical test, TLC study, solubility, melting point determination. Finally the structure was elucidated by different spectroscopic methods such as UV, IR, NMR (13 C NMR and Proton NMR) and LCMS. On the basis of this chemical and spectral evidence and upon comparison with the literature data, the isolated compound is identified .The compound was isolated first time from this plant extract.