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A total of 4 compounds have been isolated from a methanol extract of Bryophyllum daigremontianum. These compounds were identified as glut-5(6)-en-3β-ol (1), mixture of α-amyrin (2a) & β-amyrin (2b) and stigmasterol (3), by extensive analysis of NMR data and by comparison with published values. The crude extract was subjected to assay for antioxidant potential through determination of total phenolic content, membrane stabilizing and thrombolytic activities, which revealed potent antioxidant and moderate membrane stabilizing activities and inhibition of clot of rat’s blood.