Somayeh Afsah Vakili

The current investigation was delineated to find out the anticancer activity of three different formulations of Triphala and to find the best Triphala formulation for anticancer activity among the three. Aqueous extracts of Terminalia chebula, Termialia bellerica and  Phyllanthus emblica were obtained from Amruta herbals, Indore was dissolved in purified water to make Triphala 1:1:1, 1:2:3, 1:2:4 (F1, F2, F3 respectively) formulation. The number of 1x106 Ehrlich ascites carcinoma (EAC) cell was inoculated in mice. Group I served as untreated negative control while group II was considered as positive control. Group III cancerous animals received standard drug cyclophosphamide 25 mg/kg b.w., whereas group IV to VI cancerous animals were treated with F1 150, 300 and 600mg/kg b.w., group VIII to IX with F2 150, 300 and 600 mg/kg b.w and group X to XII with F3 150, 300 and 600mg/kg b.w., respectively. Different parameter such as change in body weight, determination of survival time, total ascites fluid volume, packed cell volume and haematological parameters were perceived. At the end of investigation, 6 animals from each group were immolated, blood samples were collected and WBC, RBC, Hb content was reckoned. The effect of all three formulations on reduction in body weight, tumor volume, packed cell volume, percentage increase in life span (%ILS) and haematological parameters were compared. The data revealed were suggested that all the three Triphala formulation possessed significant anticancer activity and Triphala 1:2:3 formulation at 600 mg/kg b. w., possessed maximum anticancer activity among the 9 groups compared. Key words: Triphala, Antitumor activity; Ehrlich ascites carcinoma.

This survey was designed to investigate the evaluation of synergic anticancer activity of Punica grantum L and Ziziphus mauritiana extracts by using invitro cytotoxicity assay, invitro and invivo antioxidant estimation. The invitro antioxidant activity of ethanol, aqueous and chloroform extracts of  Punica grantum L and Ziziphus mauritiana and combination of them was estimated by reducing power, diphenyl picryl hydrazyl scavenging and hydrogen peroxide scavenging assays using standard procedure, the invivo antioxidant activity was  also performed  by estimation of  Catalase and Glutathione in liver homogenate. The extracts were screened for their invitro cytotoxicity by trypan blue exclusion assay and Allium cepa root tips. The results displayed the 100µg/ml of both ethanol and aqueous extracts i.e. Punica grantum L and it’s combination with Ziziphus mauritiana having the maximum invitro antioxidant activity. Ethanol and aqueous extract of combination of Punica grantum L and Ziziphus and ethanol extract of Punica grantum L has produced the significant increase in the glutathione and catalase levels. The cytotoxic effect of ethanol extracts of Punica grantum L and Ziziphus mauritiana significantly increased in Ehrlich ascites tumour cells with an increase in concentration by trypan blue cytotoxic assay and also significant root growth inhibition in Allium cepa after 48h. The percentage decrease in mitotic index was found to be 27.28 % in 800 μg/ml of ethanol extracts of Punica grantum L and Ziziphus mauritiana. Accordingly, the synergic anticancer activity of illustrated extracts was evinced. Key words: Antioxidant, cytotoxic assay, Punica grantum L, Zizipus mauritiana