Manikanta Kumar. A

Lawsonia inermis (Lythraceae) commonly called as Henna or Mailanchi is known for its cosmetic properties. Henna is an important source of phytochemicals of immense medicinal and pharmaceutical significance such as alkaloids, naphthoquinone derivatives, aliphatic components, tannins, phenolic derivatives, coumarins, xanthones, flavanoids, therefore an effort to compile all the information reported on its phytochemical and pharmacological activities, so that interest could be diverted towards this dye herb, for the treatment and relief from various ailments and diseases. The resultant extracts were analyzed by thin layer chromatography (TLC), column chromatography, ultraviolet spectroscopy (UV), Infrared (IR) spectroscopy, and High performance liquid chromatography (HPLC) techniques UV analysis was done by diluting the extracts with their respective solvents. 2 to 3 peaks were observed for extracted samples at 212 nm, 238.60 nm and 283.30 nm in methanol extract, 241 nm in chloroform extract, 238 nm in chloroform: methanol extract and 272.20 nm in ethyl acetate extract respectively. In HPLC  compounds  methanol extract is carried out for HPLC separation, and mainly three peaks were observed at 2.057, 2.522, 4.133 min retention times, which is the confirmation of presence of three separated compounds.

A simple, rapid and specific UV spectrophotometric method with good sensitivity was developed and validated for the simultaneous determination of tamsulosin and finasteride in bulk and pharmaceutical formulations. In methanol, the lambda max of finasteride and tamsulosin was fixed as 235 and 225nm respectively, using a Shimadzu UV–Visible spectrophotometer (model UV-1800) with quartz cells. In this proposed method both these drugs obeyed linearity individually and in mixture within the concentration range of 1- 10 μg ml-1 for tamsulosin and 12.5 - 100 μg ml-1 for finasteride, with a correlation coefficient value of  0.9992 and 0.9994 for tamsulosin and finasteride respectively. The low relative standard deviation values indicate good precision and high recovery values indicate accuracy of the proposed method. The proposed method had been applied to the determination of drugs in commercial formulations. Assay results were in good agreement with label claim. The method was validated according to the ICH guidelines.