UV spectrophotometric method

A simple, rapid, precise and highly selective spectrophotometric method was developed for simultaneous estimation of Norfloxacin and metronidazole in tablet dosage form. This method involves the measurement of absorbances of Norfloxacin and Metronidazole at the wavelength of 272nm (λmax of Norfloxacin) and 334nm (λmax Metronidazole) .0.1 N NaOH was used as solvent. Linearity was observed in the concentration range of 1-10 µg/ml for Norfloxacin and 1- 19µg/ml for Metronidazole. The accuracy of the method was confirmed by recovery studies of tablet dosage forms and was found to be 101 and 102% for Norfloxacin and metronidazole respectively. The method showed good reproducibility and recovery with % RSD less than 2. The LOD of Norfloxacin and metronidazole and LOQ of Norfloxacin and metronidazole   was found to be 0.3μg/ml and 1.16μg/ml and 0.79μg/ml and 2.4μg/ml respectively. The developed method was free from interferences due to excipients present in tablets .Thus the proposed method was found to be rapid, specific, precise, accurate and cost effective quality control tool for the routine analysis of Norfloxacin and metronidazole in bulk and combined dosage form.

A simple, sensitive, accurate and precise simultaneous UV spectrophotometric method has been developed for the estimation of Ibuprofen, Paracetamol and Caffeine in tablet dosage form. The absorption maxima of the drugs were found to be 223, 248 and 272 nm for Ibuprofen, Paracetamol and Caffeine respectively, in methanol, using a Shimadzu UV–Visible spectrophotometer (model UV-1800). Ibuprofen, Paracetamol and Caffeine obeyed Beer’s law in the concentration range of 10-70 µg ml-1, 10-60 µg ml-1 and 10-70 µg ml-1 respectively. The correlation coefficient was found to be 0.999, 0.999, and 0.999 for Ibuprofen, Paracetamol and Caffeine respectively. The method was validated for various parameters according to ICH guidelines. The low relative standard deviation values indicate good precision and high recovery values indicate accuracy of the proposed method. Assay results were in good agreement with label claim.

A rapid, simple, sensitive, accurate, and precise UV spectrophotometric method has been developed for the simultaneous estimation of anti-retroviral agents lamivudine, didanosine and efavirenz in pharmaceutical dosage form. The absorption maxima of the drugs were found to be 271, 250 and 247 nm for lamivudine, didanosine and efavirenz respectively. Lamivudine, didanosine and efavirenz obeyed Beer’s law in the concentration range of 10-100 µg/ml, 10-100 µg/ml and 10-70 µg/ml respectively. The percentage recovery was within the range of 98% - 101%, indicating that insignificant interference from the other ingredients in the formulation. The above method was validated in terms of linearity, accuracy, precision, Limit of Detection (LOD), Limit of Quantification (LOQ) etc. in accordance with ICH guide lines. The developed method was free from interferences due to excipients present in tablets. The method was rapid, simple and suitable for routine quality control analysis.

A simple, rapid and specific UV spectrophotometric method with good sensitivity was developed and validated for the simultaneous determination of tamsulosin and finasteride in bulk and pharmaceutical formulations. In methanol, the lambda max of finasteride and tamsulosin was fixed as 235 and 225nm respectively, using a Shimadzu UV–Visible spectrophotometer (model UV-1800) with quartz cells. In this proposed method both these drugs obeyed linearity individually and in mixture within the concentration range of 1- 10 μg ml-1 for tamsulosin and 12.5 - 100 μg ml-1 for finasteride, with a correlation coefficient value of  0.9992 and 0.9994 for tamsulosin and finasteride respectively. The low relative standard deviation values indicate good precision and high recovery values indicate accuracy of the proposed method. The proposed method had been applied to the determination of drugs in commercial formulations. Assay results were in good agreement with label claim. The method was validated according to the ICH guidelines.