Analytical Estimation of Secondary Metabolites In Lawsonia Inermis Leaves

Lawsonia inermis (Lythraceae) commonly called as Henna or Mailanchi is known for its cosmetic properties. Henna is an important source of phytochemicals of immense medicinal and pharmaceutical significance such as alkaloids, naphthoquinone derivatives, aliphatic components, tannins, phenolic derivatives, coumarins, xanthones, flavanoids, therefore an effort to compile all the information reported on its phytochemical and pharmacological activities, so that interest could be diverted towards this dye herb, for the treatment and relief from various ailments and diseases. The resultant extracts were analyzed by thin layer chromatography (TLC), column chromatography, ultraviolet spectroscopy (UV), Infrared (IR) spectroscopy, and High performance liquid chromatography (HPLC) techniques UV analysis was done by diluting the extracts with their respective solvents. 2 to 3 peaks were observed for extracted samples at 212 nm, 238.60 nm and 283.30 nm in methanol extract, 241 nm in chloroform extract, 238 nm in chloroform: methanol extract and 272.20 nm in ethyl acetate extract respectively. In HPLC  compounds  methanol extract is carried out for HPLC separation, and mainly three peaks were observed at 2.057, 2.522, 4.133 min retention times, which is the confirmation of presence of three separated compounds.