K. Vinod kumar

The majority of pharmaceuticals used today are chiral. A brief summary of various chiral separation principles and associated separation methods is provided in this review. Liquid chromatography (LC), capillary electrophoresis (CE), high-performance liquid chromatography (HPLC), and gas chromatography (GC) are only a few of the recent advancements addressed. The creation of competent analytical techniques is essential for compliance with the numerous regulatory obligations. The rapid development of chiral separation technologies has been fueled by the current situation in which chirality factors are taken into account in therapeutic benefit and drug development. High-performance liquid chromatography (HPLC) and gas chromatography (GC) have unquestionably made important advancements in chiral chromatographic technology, opening the door to the quantification of specific enantiomers of a number of racemates in biological fluids such plasma, serum, urine, etc. Chiral compound separations and analyses are crucial in a variety of industries, such as biology, pharmaceutical manufacturing, and the creation of chemical intermediates.

A simple, accurate, precise, specific, sensitive, reproducible and Reliable RP- HPLC Method was developed for Quantitative Estimation of Metaxalone and Diclofenac potassium in Pharmaceutical Dosage Form. The developed RP- HPLC method with the mobile phase Methanol: Water (80: 20) and Qualisilgold-C18 (250Χ4.6mm, 5μm particle size) as stationary phase with a flow rate of 1.0 mL/minute by using λmax 275nm and PDA detector. Proposed method was found to be linear in the concentration range of 8.0 to 80.0 μg/mL for Metaxalone and 1.0 to 10.0 μg/mL for Diclofenac potassium respectively, and the correlation coefficient was found to be 0.9991 for both the drugs. Precision study showed that the % RSD was within the range of acceptable limits (< 2), and the % Recovery was found to be in the range of 99.29%-101.28% for Metaxalone and 99.98%-102.45% for Diclofenac potassium. The proposed method has been validated as per ICH guidelines.