Akhilesh

This study aimed to evaluate the phytochemical profile and antimicrobial potential of extracts derived from Curcuma caesia rhizomes and Nardostachys jatamansi roots. A total of 250 g of Curcuma caesia and 80 g of Nardostachys jatamansi were subjected to solvent extraction. The yields of Curcuma caesia extracts were 2.22% in petroleum ether and 7.15% in ethanol. Phytochemical screening of the ethanolic extracts confirmed the presence of several bioactive constituents, including alkaloids, flavonoids, terpenoids, tannins, phenolics, saponins, glycosides, and proteins. The total phenolic content was measured at 171 mg/g for Curcuma caesia and 393 mg/g for Nardostachys jatamansi, expressed in gallic acid equivalents. Similarly, the total flavonoid content was 175 mg/g and 410 mg/g, respectively, in terms of rutin equivalents. A polyherbal gel was formulated using these extracts, with the G3 combination formulation showing superior performance in antimicrobial activity tests, as evidenced by a larger zone of inhibition. Furthermore, dermal safety assessment revealed no skin irritation, indicating its suitability for topical application. Overall, the findings support the potential of this herbal gel as a safe and effective natural antimicrobial agent.

This study presents the analytical assay method development and validation of Itraconazole in an ointment dosage formulation by using reverse phase HPLC. The assay method development was carried out by using C18 column. In the beginning, the study was carried on Itraconazole API by taking USP method as a reference. By changing few chromatographic parameters, a symmetrical peak and a satisfactory result could able to achieve. Changes done as per USP chapter “allowable adjustment to United States Pharmacopeia method”.  The aim was to achieve an advanced chromatographic conditions on HPLC system with C18 column (150 × 4.6 mm, 5µm particle size) using mobile phase composed of acetonitrile and neutral buffer. The separation was achieved at different gradient flows for alternative methods.  The ideal wavelength was calibrated by using PDA detector and the same wavelength was used for UV- visible detector. The assay method developed was also validated with full agreement with present regulatory guidelines by applying well developed analytical method validation techniques and means which includes the parameters such as linearity, accuracy, method precision, specificity with force degradation, robustness, solution stability, system suitability. The method shows linearity over a concentration range of 10µg/ml to 250µg/ml with r²=0.999 and thus this represents the method is efficient to provide good detector response. The lower limit of detection and quantification was achieved by carrying out the studies like LOD and LOQ and the results were found to be 10µg/ml and 5µg/ml respectively.

Present study was design to investigated In vitro antioxidant activity of hydroethanolic (HEETF), methanolic (METF) and aqueous extracts (AQETF) of Thalictrum foliolosum family-Ranuculacaece. Traditionally used for jaundice, antimalarial, antipyretic, Diuretics, dyspepsia and febrifuge was found in scientific literature. The antioxidant activity (AA) was determined by the possible four complementary test assay methods namely total phenolic content, total flavonoid content, Inhibition of  2,2 diphenyl -1 picrylhydrazyl (DPPH) radicals and ABTS (2-2’- azinobis) radical scavenging activity or quenching activity .Total phenolic content were found in HEETF, METF and AQETF 43.64±24.2, 53.64± 32.8 and 55.82±30.6 respectively, result shows as mg/GAE/ g of extract. Total flavonoid content  were found in HEETF, METF and AQETF 25.43±31.3, 33.42± 29.3 and 42.67±31.8 respectively, result shows as mg/Rutin /g of extract.  In DPPH radical scavenging activity, IC50 value were found in HEETF, METF and AQETF 4.270µg/ml, 4.90 µg/ml and 5.170 µg/ml respectively, in ABTS 2-2’- azinobis radical scavenging activity value were found in HEETF, METF and AQETF  3.35µg/ml, 3.58µg/ml and  4.86 µg/m respectively. The present study revealed that hydroethanolic extract shows significant Antioxidant, Thus it provides a platform for further research, it could be a potential candidate for further drug development