A. Jerad Suresh

The present study was undertaken to determine the forced degradation of Pyrazinamide, performed by various conditions such as acid, alkali, oxidation, thermal and photolytic. The study includes both Pyrazinamide in bulk and tablet formulation. The study based on available guidelines and main reference .Pyrazinamide has a Pyrazine nucleus. It is easily hydrolyzed by acid and alkali. The assay value of degraded products measured by intraday (30mins, 60mins, 90mins) and interday (1st, 3rd, 5th day) by UHPLC. Extensive degradation was observed in alkali hydrolysis method, and the degraded products were analysed by using UHPLC. At 90mins of intraday study using 0.1M NaOH, the degradation assay value of bulk and formulation were found to be 84.50% and 83.40% respectively. Intraday study, the degradation of bulk and formulation was observed on 1st day with the assay value of 23.62% and 25.42% respectively. However complete degradation of Pyrazinamide was observed on 3rd day and 5thday. It was determined that Pyrazinamide was found to be extremely unstable under alkali condition.

The present study was to evaluate preliminary phytochemical analysis and in vitro cytotoxic activity on root extracts and fractions of Jatropha gossipiifolia (family Euphorbiaceae). The Chloroform and ethanol extract was obtained by hot continuous percolation by soxhlet apparatus. The phytochemical analysis of both the extracts shows the presence of flavonoids, alkaloids, tannins, phenolic compounds and steroids. Both the extracts subjected to in vitro cytotoxic activity against breast cancer cell line (MCF–7) cell line by MTT assay. The active extract (ethanol) was then subjected to fractionation by column chromatography by gradient elution from n hexane – ethanol. The fractions (chloroform 100%, chloroform: ethyl acetate, ethyl acetate: ethanol, ethanol 100%) The fractions were then subjected to in vitro cytotoxic activity against MCF– 7 cell line by MTT assay. The ethanol extract shows good anticancer activity. The percentage growth of MCF-7 cells in chloroform and ethanol extract treated against MCF-7 cell line was found to be 49.59 and 28.46 respectively. The EC50 value of chloroform and ethanol extract was found to be 0.00018 & 0.00055mg/ml respectively. The ethanol 100% shows good anticancer activity compared to other fractions against MCF-7 cell line with the percentage growth of 14.650 and EC50 value was found to be 0.00087mg/ml.

  A novel series of 2-substituted 4,5-diphenyl imidazoles were synthesized and investigated for their antioxidant activity and membrane stabilization activity. 2,2-diphenyl-1-picrylhydrazyl(DPPH) radical assay was carried out to evaluate the antioxidant potential of the extract. The antioxidant activity of the synthesized compounds increased in a concentration dependent manner. In DPPH radical scavenging assay the IC50 value of the compounds ranged from 40 to 200µg/ml. The membrane stabilization activity of the compounds was evaluated using human red blood cells (HRBC) membrane stabilization method. The concentration of 88.88 to 444.44µg/ml showed a dose dependent inhibition of haemolysis of erythrocytes induced by hypotonic solution. Key words: DPPH, antioxidant, membrane stabilization