Fingerprint chromatogram analysis and detection of antioxidants markers in various extracts from the leaves of Hemigraphis colorata (blume) H.G. Hallier by high performance thin layer chromatography

A simple and reliable high performance thin layer chromatographic (HPTLC) method has been developed and validated for the study of fingerprint chromatograms of the hexane , chloroform, ethyl acetate and aqueous extracts of leaves of Hemigraphis colorata (blume) H.G. Hallier .HPTLC separation of the all extracts was performed and scanned  at ultraviolet absorbance at 254 nm. A mobile phase composed of toluene: ethyl acetate: formic acid :methanol (3:6:1.6:0.4).The chromatogram obtained and the peak profile of the components collected by scanning could  made up the fingerprint of the  various  extracts from leaves of  Hemigraphis colorata and compared with antioxidants markers of flavonoids and phenolic acids. The fingerprint chromatograms had a good stability, precision, and reproducibility. The method is suitable for differentiation of extracts from the leaves of Hemigraphis colorata, and can be used as a quality control method for this plant.