A Review on Analytical Methods for Estimation of Modafinil

Modafinil is a wakefulness - promoting agent  (or eugeroic) used for treatment of disorders such as narcolepsy, shift work sleep disorder, and excessive  daytime sleepiness associated with obstructive sleep apnea.  It is sold under the brand names Alertec, Modavigil, and Provigil. Various analytical methods used for the estimation of Modafinil have been reviewed in this paper. These include Ultraviolet Spectrophotometry, RP-HPLC, Stability indicating RP-HPLC, HPTLC, Capillary zone electrophoresis, simultaneous determination by HPLC, LC-MS/MS, Liquid –liquid extraction,  HPLC , bidimensional HPLC, GC-MS and Solid-phase extraction followed by HPLC  in rat serum and urine  to determine the amount of Modafinil in bulk drug, pharmaceutical dosage form and biological fluids. These analytical methods can be used for qualitative and quantitative estimation of Modafinil in bulk, formulation and biological fluids.