Quantitative Analysis of Neem Extract Complex using Phosphatidyl choline obtained from Soya Lecithin oil

The neem tree has been known for its unique properties both in improving human health and against insects. Azadirachtin is a tri terpenoid limonoid obtained from various parts of the neem. Phosphatidyl choline is the most important phospholipid which helps in crossing the lipid barrier of the cell membrane and thus helps in easy absorption of the compound/drug. In the present work we are combining the isolated Azadirachtin and PC to form a complex, which can enhance the bio availability and easy absorption of the compound/drug. First of all, Azadirachtin is extracted from the dried neem kernel powder in a Soxhlet extractor using Di-Chloro methane as a solvent. Phosphatidyl choline is extracted and isolated from soya lecithin oil through reflux, using Methanol + Sodium hydroxide as solvent. The TLC, UV, HPLC, FTIR and DSC reports indicate the separation of the compounds from their crude forms. Azadirachtin and Phosphatidyl choline separated are refluxed together for 2 hours and dried in a Rota evaporator to form a 'Complex'. The absorbance's of Azadirachtin, Phosphatidyl choline and the Complex are measured at 220, 230 and 217nm. The qualitative analysis of Azadirachtin, Phosphatidyl choline and the Complex (1:1) were carried out by HPLC, on a C-18 column. Aceto nitryl: Methanol: 1% Triethyl amine pH 4 (60:40:1) was used as mobile phase at a flow rate of 1ml/min, at 210nm.The isolation of Phosphatidyl choline from soya lecithin oil used is a new method and the formation of Complex is an innovative work.