Standardization and Characterization of Oleo Resin of Pine obtained from Pinus roxburghii Sarg.

The present study was design to standardize and develop scientific data for identification and quality control of Oleo Resin of Pine. The proximate analysis and nature of Oleo Resin of Pine (ORP) was confirmed by organoleptic, physiochemical characterization and fluorescence analysis. The preliminary phytochemical analysis was done by qualitative chemical tests. The total chemical components were confirmed by TLC and HPTLC analysis in suitable solvent system. Identification of chemical compounds, their molecular weight and structure were detected by GC-MS and the functional groups were detected by FTIR. Thermal analysis was carried out by DSC. The organoleptic and physiochemical data showed characteristic features of ORP. The preliminary phytochemical analysis of hydroalcholic extract of ORP showed presence of carbohydrates, glycosides, alkaloid, phytosterols, terpenes, saponins and phenols. The DSC thermogram of powder of ORP showed enthalpy of transition more than zero for all the 4 endothermic peaks. The GC-MS chromatogram of ORP revealed the presence of two compounds, Longicyclene at RT 21.11 and Longifolene at RT 22.03. TLC and HPTLC fingerprinting of ORP showed separation of components in Toluene: Ethyl acetate (93:7) mobile phase at 254, 366, 550nm.The FTIR spectrogram of ORP showed the seven characteristic bands assigned to different functional groups. The observations and results of the study have provided evidence based scientific data for standardization of ORP which will serve as reference standards to establish its identity, purity and also help to minimize adulteration and substitution. Keywords: Standardization; Oleo Resin of Pine; HPTLC; GCMS; FTIR; DSC