Synergistic/Antagonistic Interactions of Antibiotics and Hot Aqueous Tinospora Cordifolia Extracts

Present study analyzes antibacterial and synergistic potential of different parts of Tinospora cordifolia hot aqueous extracts against three environmental isolates and three MTCC reference strains of K. pneumoniae, E. coli and P. aeruginosa. Antibacterial activity was observed by agar dilution method in triplicates. Synergistic potential of extracts was observed with seven different antibiotics named as Ceftiaxone (CTR), Kanamycin (K), Gentamycin (GEN), Chloramphenicol (C), Cefotaxime/Cephotaxime (CTX), Tetracycline (TE) and Nalidixic Acid (NA). Results revealed that no extracts shows any antibacterial activity, while synergistic activity was observed with various antibiotics with plant extracts. This concluds that plant extract contains antibiotic resistance inhibitor compound in it. Results show maximum synergistic activity with chloramphenicol against P. aeruginosa with all four aqueous extracts. While no synergistic or antagonistic activity was observed against K. pneumoniae with hot aqueous fruit and leaf extract with any antibiotics used. Antagonistic activity of hot aqueous leaf extract was observed with Ceftiaxone against E. coli followed by Cefotaxime against MTCC K. pneumoniae. While synergistic activity was observed with Chloramphenicol against P. aeruginosa, MTCC P. aeruginosa and Ceftiaxone against P. aeruginosa. It will be a great achievement for the researchers to test different combination of antibiotics and plant extracts without any confirmatory consideration of their individual effect in-vitro. This kind of synergistic work can lead to great evolution in pharmaceutical industries.