tannins

This work is devoted to phytochemical study of tannic extracts from the leaves of Combretum paniculatum (CP), Mallotus oppositifolius (MO) and Ximenia americana (XA), three medicinal plants of Ivorian flora, used in traditional medicine against certain types of cancer. Qualitative analysis by phytochemical screening of these extracts, performed by means of TLC, revealed in addition to hydrolysable and condensed tannins, the presence of phenolic acids and flavonoids. Total tannin contents determined by permanganometry and spectrophotometry are respectively 41.03 ± 0.77% and 12.3648 ± 0.0004% for MO; 20.03 ± 0.44% and 11.7974 ± 0.0001% for XA; 18.76 ± 0.89% and 11.6026 ± 0.0008% for CP. Condensed tannin content in XA leaves (234.135 ± 0.003 µg ECT/mg) is considerably higher than in CP (37.731 ± 0.001 µg ECT/mg) and MO (19.082 ± 0.001 µg ECT/mg). All XA extracts (aqueous and ethyl acetate) have shown good antioxidant activity with CR50s which are lower than that of vitamin C (reference antioxidant). A correlative relationship was found between tannin content and antioxidant activity.

In India, many indigenous plants are used in herbal medicine to cure diseases and heal injuries. Major pharmaceutical companies are currently conducting extensive research on plant materials gathered from the rain forests and other places for their potential medicinal value. Toddalia asiatica (L.) Lam. (Rutaceae) is widely used as a folk medicine in India. The roots are used in the treatment of indigestion and influenza and the leaves to treat lung diseases and rheumatism. The root and its bark have been used to treat fever, malaria, cholera, diarrhoea and rheumatism 1. Despite the use of this plant for such purposes, there is little information on the biochemical composition of Toddalia asiatica.  L.Var . floribunda.. This work is therefore aimed at documenting the biochemical compositions of Toddalia asiatica. L. Var . floribunda. From the earlier reports qualitative phytochemical results reveals that the methanolic stem extract of Toddalia asiatica.L. contain secondary metabolites like  Alkaloids, Flavonoids, Saponins, Steroids, Glycosides, Tannins. From the quantification result of various biochemical and phytochemical constituents like carbohydrates, proteins, total ascorbic acid, phenols, tannins and flavanoids content, it was noted that alkaloid found to be the highest with phenol as the next active constituents among the secondary metabolites in the stem. From the results it reveals Toddalia asiatica has the potential to be represented as an effective biochemical and therapeutic constituent for the future use in the medical field.