tablet dosage form

Alogliptin Benzoate is a novel hypoglycemic drug that belongs to dipeptidylpeptidase-4 inhibitor class which stimulates glucose dependent insulin release. The Present work describes development and validation of a new simple, accurate, precise and stability indicaing HPTLC method for the determination of alogliptin benzoate in tablet dosage form. The chromatographic separation was achieved by using Chloroform: Methanol 3:7 v/v as mobile phase and UV detection at 275 nm. The developed method was validated with respect to linearity, accuracy, precision, limit of detection, limit of quantitation and robustness as per ICH guidelines. The drug was subjected to stress condition of acid hydrolysis, alkali hydrolysis, photolysis, thermal degradation. Results found to be linear in concentration range of 500-2500 ng/band. The developed method can be used for the quantification of bulk drug as well as tablet dosage form

A simple, precise, economical and accurate difference spectroscopic method has been developed for the valacyclovir in bulk and in pharmaceutical dosage form. The proposed method is based on the principle that valacyclovir exhibits two different chemical forms that differs in the absorption spectra in acidic and basic solution. The absorptions were measured in acidic and basic solution separately against reagent blank. Valacyclovir has exhibited maximum absorbance at 252 and 262nm in acidic and basic solution respectively. Difference in absorbance between these two maxima was calculated to find out the amplitude. The amplitude plotted against concentration showed linear response in the concentration range of 0.5 – 2.5μg/mL with linear regression value 0.999. The proposed method was applied to pharmaceutical formulation and the common excipient present in the formulation does not interfere in the analysis of the drug. The method was validated as per ICH guidelines and statistical results of analysis were found to be satisfactory.