phytochemical and pharmacological screening.

Last few decades, research data has prompted a passionate debate as to whether oxidation, or specifically, oxidative stress mediated by free radicals/reactive oxygen species (ROS)/reactive nitrogen species (RNS), is a primary or secondary cause of many chronic diseases. Lagenaria siceraria is fair source of ascorbic acid, beta carotene and different phytochemicals to heal many disorders. It shows ribonucleolytic, antihyperlipidemic, hepatoprotective, antioxidant, immunomodulatory, cardiotonic activity. The collection and identification of juice of Lgenaria siceraria and preparation of four fractions of concentrated juice were carried out and preliminary phytochemical and pharmacological screening of same fractions was done. Preliminary pharmacological screening of each fraction was investigated to find out desired in-vitro antioxidant activity. Aqueous fraction showed better yield because of polarity of compound. Preliminary phytochemical screening of four different fractions had various phytochemicals like steroids, flavonids, glycosides, saponins, etc. As we had followed the biological activity guided isolation of active constituents, the fractions were evaluated for antioxidant activity. Isolated aliphatic and saponins moiety may be responsible for the antioxidant activity.