histopathology

The main aim of this study was to evaluate the effect of Colgate Dental Cream on Catfish. Investigation was carried out using Catfish weighing about 475-525 g. Fishes were maintained for 24 h in the water, dissoved with 100 mg of Colgate Dental Cream.  Reduced serum total protein, albumin, glucose and urea, concomitant with a reduced concentration chloride ions were observed. Enzymes such as alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP) enzyme activities were changed significantly. In addition, structural architecture of  liver, heart, gills, muscle and kidney were also changed. Biochemical and histopathological changes in the catfish were due to the fact that there was an increased demand for energy under stress to cope up with the detrimental effects of the dental cream.

Peucedanum grande has been found to be associated with the multiple therapeutic properties. In the present study, we have used P. grande as an ameliorating agent against nephrotoxic effects of Mercuric chloride (HgCl2). The rats were given pretreatment of P. grande orally at a dose of 60 and 120 mg/kg body weight for five consecutive days. Mercury chloride 4 mg/kg body .wt was used as renal toxicant, and injected subcutaneously in the neck region in a volume of 1 ml/kg. The modulatory effects of P. grande on HgCl2 induced nephrotoxicity was investigated by assaying oxidative stress biomarkers, lipid peroxidation, serum kidney toxicity markers and by histopathological examination of kidney. The HgCl2 induced nephrotoxicity by depleting antioxidant levels, elevating the level of serum creatinine and BUN, as well as damaging the normal architecture of kidney. P. grande pretreatment prevented deteriorative effects induced by HgCl2 through a protective mechanism that involved reduction of increased oxidative stress as well as by restoration of histopathological change against HgCl2 administration.