UV-Vis Spectrophotometer

The present study was undertaken to develop and validate a simple, accurate, precise, reproducible and cost effective UV-Visible spectrophotometric method for the estimation of cefpodoxime proxetil in bulk and pharmaceutical formulation. The solvent used throughout the experiment was methanol and water. Absorption maximum (λmax) of the drug was found to be 231 nm. The quantitative determination of the drug was carried out at 231 nm and Beer’s law was obeyed in the range of 5-25μg/mL. The method was shown linear in the mentioned concentrations having line equation y = 0.0331x + 0.0151 with correlation coefficient R2of 0.9998. The recovery values for cefpodoxime proxetil ranged from 99.80% - 100.02%.The percent relative standard deviation (RSD %) of interday precision range was 0.118 – 0.181% and intraday precision range was 0.139 – 0.446%. The limit of detection and limit of quantification was 0.081μg/mL and 0.121μg/mL. The percent relative standard deviation of robustness and ruggedness of the method was 0.126 – 0.313%.Cefpodoxime proxetil content in two pharmaceutical dosage forms (tablet and suspension) were determine which were in good agreement with the label claims with RSD value of 0.02% for tablet and 0.01% for suspension. Hence, proposed method was precise, accurate and cost effective. This method could be applicable for quantitative determination of the bulk drug as well as dosage formulation.