UV-Spectroscopy

A simple, precise and accurate UV Spectrophotometric method has been developed and validated for estimation of Sildenafil in bulk and tablet dosage form. In this method Sildenafil shows λmax at 290nm using 0.1N HCl as a solvent and calibration graphs were plotted over the concentrations ranging from 5 to 30μg/ml of Sildenafil with correlation coefficient 0.999. The proposed method was validated as per ICH Q2 (R1) guidelines for precision, linearity, accuracy and recovery. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.113μg/ml and 0.343μg/ml respectively by simple UV spectroscopy. The proposed method was validated.