Torsemide

A simple, accurate and stability indicating high performance liquid chromatographic (HPLC) method was developed for the simultaneous estimation of Torsemide and Spironolactone in combined dosage form. Isocratic RP-HPLC separation was achieved on Kromasil RP- C18 column (250mm×4.6mm; 5µm) using methanol: acetonitrile: water in the ratio of 50:30:20 (v/v), pH6.8, at flow rate of1.0ml/min at ambient temperature. Quantization was achieved by UV detection at 235nm over the concentration range of 10-60μg/ml for torsemide and 25-150μg/ml for spironolactone with percentage recoveries of range 99.688-101.792 and 98.282-101.811for torsemide and spironolactone respectively. Different stress degradation studies like acidic, alkaline, peroxide, thermal etc were measured for both standard drugs and results found that the stress degradation conditions doesn’t affect the elution of the both the drugs and hence the developed method was found to be stability indicating method.