Tablet formulation

A simple, sensitive and specific HPLC method with UV detection was developed for the estimation of irbesartan in tablet dosage form. Separation was achieved by Lithosphere 100 RP-18e, 5µ column having 250x4.0 mm internal diameter with mobile phase containing phosphate buffer and acetonitrile in 50:50 (v/v) with an ambient temperature. The flow rate was 0.8mL min-1 and eluent was monitored at 220 nm. The proposed method was found to be rectilinear over theconcentration range of 20µg/ml to 60µg/ml. This method was validated for system suitability, Specificity, Linearity, Precision, Accuracy, Range, Stability studies, Ruggedness, Robustness and Filter validation.  The results of all the validation parameters were well within their acceptance values. Therefore the proposed method can be used for routine analysis of estimation of Irbesartan in its tablet formulation

A simple, rapid and specific RP-HPLC method has been developed and validated for determination of Carvedilol in bulk and tablet formulations. Chromatographic separation was performed by Phenomenex Luna C-18 (250 x 4.6mm, 5μm particle size) column with a mobile phase consisting of a mixture of phosphate buffer, acetonitrile and methanol in the ratio (30:45:25 v/v/v), pH adjusted to 4.8 with ortophosphoric acid. The mobile phase was was filtered through a 0.45μ cellulose nitrate filter, sonicated for 15 min and delivered at a flow rate of 1ml/min. Detection was performed at a wave length of 241 nm at ambient temperature. Linearity was obtained in a concentration range of 30 to130 µg/ml with a correlation coefficient (r2) of 0.999. The limit of detection and limit of quantification were 1.08 and 3.24 μg/ml, respectively. No interference of excipients in determining tablet formulation; identical results were obtained like that of the standard sample.  The proposed RP-HPLC method is simple, accurate, precise, rapid and economical to be employed for routine analysis of carvedilol in pharmaceutical dosage forms.