Spectrophotometric method

Sensitive spectrophotometric method is described for the determination of tetracycline HCl in bulk and in pharmaceutical formulations. The method is based on the coupling of tetracycline HCl with diazotized 8-hydroxy quinoline reagent in basic medium at room temperature to form a reddish to brown mono azo dye soluble in water with maximum absorption at 395 nm. The reaction was followed up spectrophotometrically by measuring the increase in absorbance at 395 nm. The analytical performance of the method, in terms of accuracy and precision, was statistically validated; the results were satisfactory. The calibration graph is linear in the concentration range 1-20 μg ml-1 ,with 0.005 μg ml-1 detection limit and 0.324 μg ml-1 , 0.980 μg ml-1  Limit of detection(LOD) and Limit of quantification(LOQ) respectively. The method has been successfully applied to the determination of the studied drugs in commercial pharmaceutical formulations.

  A rapid and sensitive UV-Visible spectroscopic method was developed for the estimation of cefuroxime in pure and its Pharmaceutical formulations. The method was based on the measurement of absorbance of Cefuroxime active moiety of Cefuroxime tablet at 277 nm using methanol as solvent. The absorbance was found to increase linearly with increase in concentration of Cefuroxime which was corroborated by correlation coefficient values. The standard solution of Cefuroxime obeyed Beer’s law over the concentration range of 9.20–27.60 µg/mL. The method is linear (from 9.20-27.60 µg/mL) with an R2 of 0.999, accurate (% recovery 100.56%) and precise (% RSD 0.316%). The method is specific and robust for Cefuroxime. Key words: Cefuroxime, Spectrophotometric method, validation, accuracy