Sparfloxacin

Mannich bases of sparfloxacin were synthesized from the reaction of 5-acetylamino-1-cyclopropyl-7-(3΄,5΄-dimethyl-piperazin-1-yl)-6,8-difluoro-4-oxo1,4-dihydro-quinoline-3-carboxylic acid with formaldehyde and several isatin derivatives. The structures of the synthesized compounds were elucidated from the IR, 1H NMR, 13C NMR and FAB Mass data. The synthesized compounds were evaluated for the antibacterial, antitubercular and anticancer activity. The compounds showed good activity against Gram-positive bacteria and moderate activity against tested Gram-negative bacteria. The MIC for the compounds against M. tuberculosis H37Rv strain was 50 µg/mL. The most potent compound in this series 3c exhibited enhanced antibacterial activity than sparfloxacin against S. aureus, Bacillus Sp while the anticancer activity was in the range of 18.31- 23.61 µg/mL.

The present manuscript describes simple, sensitive, rapid, accurate, precise and economical Q-absorbance ratio method for the simultaneous determination of Sparfloxacin and Dexamethasone. Absorbance ratio method uses the ratio of absorbances at two selected wavelengths, one which is an isoabsorptive point and other being the λ-max of one of the two components. Sparfloxacin and Dexamethasone show an isoabsorptive point at 271.2 nm in methanol. The second wavelength used is 241.6 nm, which is the λ-max of Dexamethasone  in methanol. The linearity was obtained in the concentration range of 3-18 μg/ml for Sparfloxacin and 1-6 μg/ml  for Dexamethasone. The concentrations of the drugs were determined by using ratio of absorbances at isoabsorptive point and at the λ-max of Dexamethasone. The method was successfully applied to pharmaceutical dosage form because no interference from the preservative was found. The suitability of this method for the quantitative determination of Sparfloxacin and Dexamethasone was proved by validation. The proposed method was found to be simple and sensitive for the routine quality control application of Sparfloxacin and Dexamethasone in Eye/Ear drops. The results of analysis have been validated statistically and by recovery studies.