Polyherbal formulation

Use of herbal medicines is increasing day by day. Herbal medicine has become popular form of healthcare. The consumption of plant based medicines and other botanicals in the west have increased manifold in recent years. Application of modern scientific knowledge coupled with sensitive analytical technique is important for the quality evaluation and standardization of polyherbal formulations. Mucuna pruriens, an important medicinal plant with wide medicinal properties, is frequently used in a large number of traditional herbal preparations. The present study deals with development and validation of RP-HPLC method for the quantitative estimation of L-DOPA from polyherbal formulations. The method involves Chromatographic separation was performed with SHIMADZU HPLC (Model no. LC- 20 AD) equipped with isocratic pump and manual injector (20μl). Spinchrom chromatographic software was used for data acquisition. RP- C18 (250 mm X 4.6 mm X 5 micron) column was used for analysis. Mobile phase comprising of methanol and water in ratio of 20:80 v/v was filtered through 0.45 micron in membrane filter (Millipore) and degassed by sonication; flow rate of 1ml/min was maintained throughout the run. Column effluent was monitored at 280 nm with variable wavelength UV detector. Thus this developed HPLC method could be further used for the determination of L-dopa in the polyherbal formulations.

Wounds are the result of injuries to the skin that disrupt the other soft tissue. Healing of a wound is a complex and protracted process of tissue repair and remodeling in response to injury. Various plant products have been used in treatment of wounds over the years. Wound healing herbal extracts promote blood clotting, fight infection, and accelerate the healing of wounds. Phytoconstituents derived from plants and identified and screened for antimicrobial activity, and the ratio was selected for the preparation of ointment. The in vitro assays are useful, quick, and relatively inexpensive. The wound healing efficacies of various herbal extracts have been evaluated in CAM assay in in-vitro model. CAM  was used to assess the angiogenic activity of polyherbal formulation. Angiogenesis plays an important role in wound healing and newly formed blood vessels comprise 60% of the repair tissue. Neovascularization helps hypoxic wounds to attain the normoxic conditions.  The CAM assay was done in prepared Polyherbal formulation , control and standard. As shown in the figure below the Prepared polyherbal formulation possess the good growth of blood vessels as compared to control and standard. This observation indicates that the developed veterinary polyherbal formulation has good angiogenic property. Improved angiogenesis, therefore, would be contributing significantly to wound healing activity of polyherbal formulation.