Liquid chromatography

A new, simple, rapid, selective, precise and accurate isocratic reverse phase ultra performance liquid Chromatography assay method has been developed for estimation of Lamivudine in tablet formulations. The separation was achieved by using column Acquity UPLC BEH Phenyl (100×2.1mm, 1.7µm), in mobile phase consisted of pH 3.8 ammonium acetate buffer and methanol. The flow rate was 0.5mL.min-1 and the separated Lamivudine was detected using UV detector at the wavelength of 277 nm. The retention time of Lamivudine was noted to be 2.50 min respectively, indicative of rather shorter analysis time. The method was validated as per ICH guidelines. The proposed method was found to be accurate, reproducible, and consistent.

A simple, precise, novel stability-indicating method was developed and validated for the estimation of Bendamustine in pharmaceutical forms. The Assay method was developed by using a detector set at 235nm and reverse phase c18 column (Inertsil ODS-2,150mmx4.6mm, 5 μm).The mobile phase consisted of 0.1%Trifluoroacetic acid: Acetonitrile 70:30 (v/v) in isocratic flow rate at 1.5mL/min for 10minutes.Sample cooler temperature and Column oven temperature were monitored in the method at 5°c and 25°c Respectively. The retention time of bendamustine was 4.8min.The proposed method was achieved and validated with respect to precision, specificity, accuracy, linearity, range, Analyte Stability, Filter variability, Robustness. The %Recovery of Bendamustine at Each level in formulations was found to be in a range of 98-102%. The linearity of the assay was established in the range of 5.053 to 75.793 µg/mL with correlation coefficient (R2) > 0.9999. The limits of detection and quantification were 0.03 and 0.05mg/mL, respectively. Specificity of the assay demonstrates no interference from and degrading products formed by alkaline, acidic, oxidative, UV light and high temperature (Thermal) conditions. This method showed more accurate, rugged and reliable determination of Bendamustine for drug stability assay in pharmaceutical studies.

The present investigation was carried out for phytochemical profile from aerial parts of methanolic extracts of Stephania wightii by using Liquid Chromatography-Electronic Spray Ionization-Mass spectrometry (LC-ESI-MS). The results confirmed the presence of eighteen compounds in the crude extract and the pharmacological activities of the identified compounds were listed. The presence of various bioactive compounds justifies the use of the whole plant for various ailments by traditional practitioners.