Indapamide

Rapid and accurate High performance liquid chromatography method is described for Simultaneous estimation of Perindropil and Indapamide from the combination tablet dosage form. The separation of two drugs was achieved on Phenomenax (C18) (4.6mm x 100mm, 3.5 mm) column. The mobile phase consists of Acetonitrile : Buffer Orthophosphoric acid 0.1% in the ratio of 40:60. The detection was carried out at a wavelength 230 nm. The method was validated for system suitability, linearity, accuracy, precision, robustness and stability of sample solution. The linear ranges for Perindropil and Indapamide were 8-40 μg/mL, 2.5- 12.5 μg/mL respectively with good recoveries i.e. 100.5% to 100.3%.

The manuscript describes validated Reverse Phase High Performance Liquid Chromatographic (RP-HPLC) method for the simultaneous estimation of Perindopril Erbumine and Indapamide in combined dosage form. The RP-HPLC separation was achieved on Intersil ODS C18 column (250 mm x 4.6 mm i.d., 5 µm particle size) at 40oC using mobile phase Acetonitrile : Acid Phthalate Buffer (pH 3.0 ± 0.05) (50 :50, v/v) at flow rate 1.0 mL/min. Quantification was achieved with photodiode array (PDA) detection at 240 nm over the concentration range of 10 - 50 µg/mL for Perindopril Erbumine and 3 - 15 µg/mL for Indapamide with mean recovery of 100.09 ± 0.52 % and 100.13 ± 0.09 % for Perindopril Erbumine and Indapamide, respectively. These methods were found to be simple, sensitive, accurate, precise and economical and applicable for the simultaneous determination of Perindopril Erbumine and Indapamide in combined dosage form.

The manuscript describes validated simultaneous equation method for the simultaneous estimation of Amlodipine Besylate and Indapamide in combined dosage form. Simultaneous equation method was based on the estimation of both the drugs at two wavelengths i.e. absorption maxima of both drugs. An absorption maxima of Amlodipine besylate was 240 nm and absorption maxima of Indapamide was 215 nm in methanol. The linearity was obtained over the concentration range of 10 – 30 µg/mL for amlodipine besylate and 2 – 10 µg/mL for Indapamide with mean recovery of 99.45 ± 0.84 % and 99.46 ± 0.33 % for Amlodipine Besylate and Indapamide, respectively. These methods were found to be simple, sensitive, accurate, precise and economical and applicable for the simultaneous determination of Amlodipine Besylate and Indapamide in combined dosage form.