In Vivo & In Vitro Antioxidant assays

The present study was investigated the antioxidant activities of the various extracts of Aegle Marmelos (AM) belongs to family Rutaceae. The antioxidant activities of extracts have been evaluated by using a range of in vitro assays and in vivo hepatoprotective model. In vitro Antioxidant activity was evaluated by percentage inhibition in different assays  including  2, 2’-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical scavenging assay hydrogen peroxide radical scavenging assays, reducing power capacity. The extract exhibited potent antioxidant activity compared to known antioxidant. The extracts of A.Marmelos were tested for in vivo efficacy by carbon tetrachloride (CCl4) induced liver damage rats in hepatoprotective model. The in vitro antioxidant activities of extracts showed significant activities on reducing power, DPPH, hydroxyl radical and hydrogen peroxide nearer to control group based on IC50 values. Oral administration of various extracts of A.Marmelos resulted in significant improvement on the levels of malondialdehyde (MDA) and superoxide dismutase (SOD), Catalse (CAT) in liver homogenate & Significant increase in level of MDA which was intoxicated by CCl4. CCl4 produced significant alteration of serum marker enzymes, total bilirubin, total protein and liver weight. The extracts significantly restored of these values towards normal compared to control group. Due to its natural origin and potent free radical scavenging ability A.Marmelos could be used as a potential preventive intervention for free radical mediated diseases. Key words: DPPH, SOD, CAT, MDA, In Vivo & In Vitro Antioxidant assays