Human RBC Stabilization

The present study was focused to assess traditional claims of Sargassum ilicifolium Turner C.Agardh towards anti-inflammatory and antioxidant property by invitro studies. In this study 70% of ethanolic extract of sargassum ilicifolium was prepared and subjected to preliminary phytochemical constituent screening followed by invitro antioxidant activity byDPPH assay using concentration 25-200µg/ml and invitro anti-inflammatory studies by Human RBC stabilization method using the concentration of 12.5-200µg/ml. The phytochemical analysis revealed the presence of alkaloids, terpenioids, steroids, phenolic compounds, fats, oils, and tannins. In DPPH assay the free radical scavenging activity of the extract was determined by its reduction in yellow colored reduced DPPH formation and IC50  value was found to be 164.98µg/ml and invitro HRBC anti-inflammatory study, the extract was effective in inhibiting the hemolysis induced by hypotonic solution added to RBC cells and % reduction of haemolysis was found to be 52.08% at concentration of 12.5µg/ml and 16.86% for 200µg/ml and standard drug Diclofenac showed 32.68 and 4.28 % reduction of heamolysis at concentration of 12.5 and 200µg/ml respectively. The result shown effective action in dose dependent manner and was statistically proved.