High performance thin layer chromatography (HPTLC)

A new simple, accurate, precise and selective stability indicating high performance thin layer chromatographic method has been developed and validated for estimation of Orlistat Tablet.T he mobile phase selected was Toluene: Methanol(8:2v/v) with UV detection at 211nm.The retention factor for Orlistat was found to be 0.60±0.02. The method was validated with respect to linearity, accuracy, precision and robustness as per the ICH guidelines. The drug were subjected to stress condition of hydrolysis (acid, base), oxidation, photolysis and thermal degradation. Results found to be linear in concentration range of 6000-36000 ng/band. The thermal method has been successfully applied for the analysis of drug in pharmaceutical formulation. The % assay (Mean ± S.D) was found to be 99.30±1.10.The developed method can be used for checking the stability of  Orlistst in bulk drug and pharmaceutical dosage form.

Analysis of pharmaceutical and neutral compound and newer drugs commonly used in all the stages of drug discovery and development process. High performance thin layer chromatography (HPTLC) is one of the easiest instrumental technique based on the full capabilities of thin layer chromatography.it is still increasingly finding its way in pharmaceutical analysis with the advancements in the stationary phase and the introduction of the densitometers as detection equipment and the technique achieves high precision and trueness comparable to high performance liquid chromatography (HPLC).