High Performance Thin Layer Chromatography (HPTLC)

Constipation is a common problem in all types of ages. Cassia angustifolia (Senna) extracts contain anthranoids that are commonly used for constipation and are well known for their laxative properties. Senna leaves or pods are highly rich with Sennosides content A, B, C & D. These Sennosides are hydroxyanthracene glycosides, which work miraculously as a laxative. In the present study, Senna extract from different vendors with variable strengths of sennosides A&B were procured and the relation between residue on ignition (ROI) with that of metals and sennosides A&B was studied.  The optimized concentration of senna extracts containing different sennosides A&B content were analyzed on HPTLC to determine the ratios of sennosides  A&B  from the total sennosides as well as its relation with residue on ignition (ROI).  Residue on ignition (ROI) was analysed gravimetrically while content of Calcium was calculated by Complexometric method. Iron content was carried out spectrophotometrically and Sennosides A&B content was carried out using HPTLC. The process control of extraction can be monitored on the basis of ROI. Specified Sennosides and ROI value together can be used in quality specification. Batch to batch variation with respect to efficacy can be avoided by determining the fixed quality norms for content of Sennosides, ratio of sennosides A&B, ROI value and content of Calcium.

A simple and sensitive stability indicating HPTLC method has been developed and validated for estimation of Palonosetron hydrochloride. Separation of the drug was carried on aluminium plates precoated with silica gel 60 F254 using Ethyl acetate: Methanol: Triethylamine (6:3:1 v/v/v) as mobile phase. The retention factor (Rf) for Palonosetron hydrochloride was found to be 0.50 ± 0.04. The detection was carried at 242 nm. Stress testing of Palonosetron hydrochloride was carried out according to the International conference on harmonization (ICH) guideline Q1A (R2). The drug was subjected to acid, base, neutral hydrolysis, oxidation, thermal degradation and photolysis. Palonosetron hydrochloride showed considerable degradation under oxidative condition. The method was successfully validated according to ICH guidelines Q2 (R1). The data of linear regression analysis indicated a good linear relationship over the range of 250–1500ng/band concentrations with correlation coefficient 0.994. The accuracy of the method was established based on the recovery studies. The LOD and LOQ were 11.81ng/band and 35.78ng/band respectively.