Fluoxetine HCl

In-situ gelling system is a process in which a solution form converted into the gel form after the formulation has been applied at the site. In-situ gel is helpful to produce sustained release of drug. Fluoxetine HCl is a selective serotonin reuptake inhibitor. The current study deals with the preparation, evaluation and optimization of In-situ gel of Fluoxetine HCl for intranasal delivery for the effective treatment of depression. The In-situ gel of Fluxetine HCl was prepared by cold-technique and evaluated for its physical appearance, viscosity, Gel forming temperature, Melting temperature, pH, Spreadability, Swelling index, Drug content and In-vitro release study. In-vitro drug release for optimized formulation was found to be 95.55% of F2 and pH, Drug content, Spreadability, Gelling temperature, Melting temperature were found to be 6.3, 97.6%, 8.0 gmcm/sec, 330C, 790C respectively. The study clearly demonstrated that the Fluoxetine HCl can be successfully delivered through nasal route by preparing in-situ gel. Gel is non-irritating when delivered through nasal route.

Simple, accurate and precise and economical UV–spectroscopy method have been developed and validated for the estimation of Tadalafil and Fluoxetine HCl in a synthetic mixture. Tadalafil is used in treatment of Erectile Dysfunction and Fluoxetine HCl is used in depression. The Tadalafil and Fluoxetine HCl stock solutions are prepared in methanol solution. At zero crossing point (ZCP) of Tadalafil (230nm) Fluoxetine HCl showed a measurable derivative absorbance, whereas at zero crossing point (ZCP) of Fluoxetine HCl (235nm) Tadalafil showed a appreciable derivative absorbance value. The Tadalafil and Fluoxetine HCl are linear in concentration range of 5-25 μg/ml. Developed method was validated according to the ICH Q2 (R1) guidelines. The precision were found within limits (RSD< 2%). Accuracy were determined by recovery studies and showed % recovery between 97 to 100 % for both the drugs Tadalafil and Fluoxetine HCl. The LOD and LOQ values of Tadalafil at ZCP 230 nm were found to be 0.24 and 0.74 correspondingly and for Fluoxetine HCl at ZCP235nm were found to be 0.29 and 0.89 correspondingly.