Clinical Chemistry.

Derivative spectroscopy is one of the important technique for as resolution of multi component systems. Many of the modern pharmaceutical formulations are so complex because they contains  number of excipients such as diluents, disintegrating agents, stabilizers, coloring agents or dyes, flavors etc. along with the active ingredient. Derivative technique is becoming increasingly popular in analytical spectrophotometry as a background correction and as a resolution enhancement technique. Derivative spectroscopy is a  technique which offers an alternative approach to the enhancement of sensitivity and specificity in mixture analysis. Derivative spectrophotometry is a technique which is based on derivative spectra of a basic, zero-order spectrum. Derivative spectroscopy is widely used in different fields of the analysis.